Menu

 

The NASA Task Book
Advanced Search     

Project Title:  Integrated Physiological Responses of CNS and Muscle in Drosophila and C. elegans Along a Gravity Continuum Reduce
Images: icon  Fiscal Year: FY 2023 
Division: Space Biology 
Research Discipline/Element:
Space Biology: Cell & Molecular Biology   | Animal Biology: Invertebrate  
Start Date: 01/01/2022  
End Date: 12/31/2024  
Task Last Updated: 11/04/2022 
Download report in PDF pdf
Principal Investigator/Affiliation:   Ocorr, Karen  Ph.D. / Sanford Burnham Prebys Medical Discovery Institute 
Address:  Center for Genetic Disorders and Aging Research 
10901 N Torrey Pines Rd 
La Jolla , CA 92037-1005 
Email: kocorr@sbpdiscovery.org 
Phone: 858-692-0051  
Congressional District: 49 
Web:  
Organization Type: NON-PROFIT 
Organization Name: Sanford Burnham Prebys Medical Discovery Institute 
Joint Agency:  
Comments:  
Co-Investigator(s)
Affiliation: 
Iyer, Janani  Ph.D. NASA Ames Research Center 
Szewczyk, Nathaniel  Ph.D. Ohio University 
Costes, Sylvain  Ph.D. NASA Ames Research Center 
Mhatre, Siddhita  Ph.D. NASA Ames Research Center 
Key Personnel Changes / Previous PI: None
Project Information: Grant/Contract No. 80NSSC22K0278 
Responsible Center: NASA ARC 
Grant Monitor: Griko, Yuri  
Center Contact: 650-604-0519 
Yuri.V.Griko@nasa.gov 
Unique ID: 14765 
Solicitation / Funding Source: 2020 Space Biology NNH20ZDA001N-SB E.12. Flight/Ground Research 
Grant/Contract No.: 80NSSC22K0278 
Project Type: FLIGHT,GROUND 
Flight Program: ISS 
No. of Post Docs:
No. of PhD Candidates:  
No. of Master's Candidates:
No. of Bachelor's Candidates:
No. of PhD Degrees:  
No. of Master's Degrees:  
No. of Bachelor's Degrees:
Space Biology Element: (1) Cell & Molecular Biology
(2) Animal Biology: Invertebrate
Space Biology Cross-Element Discipline: (1) Musculoskeletal Biology
(2) Neurobiology
Space Biology Special Category: None
Task Description: Our studies will use the fruit fly Drosophila and the worm Caenorhabditis elegans (C. elegans) to identify conserved mechanisms underlying the oxidative stress response to altered gravity. Spaceflight induces alterations in somatic/cardiac muscle, as well as in the brain. Many of these changes mirror those induced by long-term bed-rest on Earth and with age. We will use functional, structural, and molecular biological techniques to identify common genetic and molecular components that mediate the effect of microgravity, lunar gravity, and Mars gravity on organ function. The use of two different genetic model organisms will allow us to identify common targets across species that can be exploited to mitigate negative health effects of long duration space habitation and perhaps provide therapies to combat muscle wasting and neurodegeneration on Earth. We will also compare the changes in these organisms with published changes in humans subjected to bed-rest and spaceflight.

Research Impact/Earth Benefits: Understanding the effects of reduced gravities on mitochondrial structure/function and protein homeostasis will provide targets for countermeasures to aid astronauts’ muscle and neuronal function for extended stays on the lunar surface and eventually on Mars. It will also provide insights into the cellular mechanisms underlying sarcopenia and muscle wasting- thus providing targets for clinical therapies.

Task Progress & Bibliography Information FY2023 
Task Progress: We have met with our NASA managerial team and completed the Experiment Requirements Document. This is an important first step to identify hardware and other requirements that will be needed for flight.

In co-ordination with Co-Investigators (Co-Is) at NASA Ames Research Center (NASA - Ames), we are generating and testing the fly reagents needed for the experiments. These include a line of flies with a mitochondrial Green Fluorescent Protein (GFP) reporter and an ATG8-mCherry reporter that will allow us to monitor mitochondrial structure and autophagy respectively. We are also working to generate a C. elegans line with equivalent reporters. Toward this end we have brought on board a collaborator, Assistant Professor Caroline Kumsta, who will provide one of the reagents for the C. elegans experiment and will assist in combining that line with an available mitochondrial reporter line.

We are conducting preliminary tests to see if we will be able to adapt new state-of the art technology to the gene expression portion of the experiment. Toward this end we have brought on board a second collaborator, Research Assistant Professor Georg Vogler, to explore the possibility of using a new single cell RNA sequencing technology that is becoming a new standard for gene expression technology.

Bibliography Type: Description: (Last Updated: ) 

Show Cumulative Bibliography Listing
 
 None in FY 2023
Project Title:  Integrated Physiological Responses of CNS and Muscle in Drosophila and C. elegans Along a Gravity Continuum Reduce
Images: icon  Fiscal Year: FY 2022 
Division: Space Biology 
Research Discipline/Element:
Space Biology: Cell & Molecular Biology   | Animal Biology: Invertebrate  
Start Date: 01/01/2022  
End Date: 12/31/2024  
Task Last Updated: 01/04/2022 
Download report in PDF pdf
Principal Investigator/Affiliation:   Ocorr, Karen  Ph.D. / Sanford Burnham Prebys Medical Discovery Institute 
Address:  Center for Genetic Disorders and Aging Research 
10901 N Torrey Pines Rd 
La Jolla , CA 92037-1005 
Email: kocorr@sbpdiscovery.org 
Phone: 858-692-0051  
Congressional District: 49 
Web:  
Organization Type: NON-PROFIT 
Organization Name: Sanford Burnham Prebys Medical Discovery Institute 
Joint Agency:  
Comments:  
Co-Investigator(s)
Affiliation: 
Iyer, Janani  Ph.D. NASA Ames Research Center 
Szewczyk, Nathaniel  Ph.D. Ohio University 
Costes, Sylvain  Ph.D. NASA Ames Research Center 
Mhatre, Siddhita  Ph.D. NASA Ames Research Center 
Project Information: Grant/Contract No. 80NSSC22K0278 
Responsible Center: NASA ARC 
Grant Monitor: Griko, Yuri  
Center Contact: 650-604-0519 
Yuri.V.Griko@nasa.gov 
Unique ID: 14765 
Solicitation / Funding Source: 2020 Space Biology NNH20ZDA001N-SB E.12. Flight/Ground Research 
Grant/Contract No.: 80NSSC22K0278 
Project Type: FLIGHT,GROUND 
Flight Program: ISS 
No. of Post Docs:  
No. of PhD Candidates:  
No. of Master's Candidates:  
No. of Bachelor's Candidates:  
No. of PhD Degrees:  
No. of Master's Degrees:  
No. of Bachelor's Degrees:  
Space Biology Element: (1) Cell & Molecular Biology
(2) Animal Biology: Invertebrate
Space Biology Cross-Element Discipline: (1) Musculoskeletal Biology
(2) Neurobiology
Space Biology Special Category: None
Task Description: Our studies will use the fruit fly Drosophila and the worm Caenorhabditis elegans (C. elegans) to identify conserved mechanisms underlying the oxidative stress response to altered gravity. Spaceflight induces alterations in somatic/cardiac muscle, as well as in the brain. Many of these changes mirror those induced by long-term bed-rest on Earth and with age. We will use functional, structural, and molecular biological techniques to identify common genetic and molecular components that mediate the effect of microgravity, lunar gravity, and Mars gravity on organ function. The use of two different genetic model organisms will allow us to identify common targets across species that can be exploited to mitigate negative health effects of long duration space habitation and perhaps provide therapies to combat muscle wasting and neurodegeneration on Earth. We will also compare the changes in these organisms with published changes in humans subjected to bed-rest and spaceflight.

Research Impact/Earth Benefits:

Task Progress & Bibliography Information FY2022 
Task Progress: New project for FY2022.

Bibliography Type: Description: (Last Updated: ) 

Show Cumulative Bibliography Listing
 
 None in FY 2022