Project Title:  Using Water Bears to Identify Biological Countermeasures to Stress During Multigenerational Spaceflight--80NSSC20K0283 Reduce
Images: icon  Fiscal Year: FY 2020 
Division: Space Biology 
Research Discipline/Element:
Space Biology: Cell & Molecular Biology   | Animal Biology: Invertebrate  
Start Date: 11/13/2019  
End Date: 11/12/2021  
Task Last Updated: 07/24/2020 
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Principal Investigator/Affiliation:   Boothby, Thomas  Ph.D. / University of Wyoming 
Address:  1000 E. University Ave., Department #3944 
Laramie , WY 82071 
Congressional District:
Organization Type: UNIVERSITY 
Organization Name: University of Wyoming 
Joint Agency:  
Comments: NOTE: Previously at University of North Carolina until fall 2019.  
Project Information: Grant/Contract No. 80NSSC20K0283 
Responsible Center: NASA ARC 
Grant Monitor: Griko, Yuri  
Center Contact: 650-604-0519 
Solicitation: 2014 Space Biology Flight NNH14ZTT001N 
Grant/Contract No.: 80NSSC20K0283 
Project Type: FLIGHT 
Flight Program: ISS 
No. of Post Docs:  
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Space Biology Element: (1) Cell & Molecular Biology
(2) Animal Biology: Invertebrate
Space Biology Cross-Element Discipline: None
Space Biology Special Category: None
Task Description: NOTE: Continuation of "Using Water Bears to Identify Biological Countermeasures to Stress During Multigenerational Spaceflight," grant NNX15AB44G, when Principal Investigator was at University of North Carolina.

For most organisms the stresses associated with spaceflight induce a variety of detrimental effects. To foster a safe and productive long-term human presence in space, therapies and countermeasures to spaceflight-induced stress should be developed. Tardigrades (water bears) are polyextremophiles that have evolved to tolerate multiple extreme environments, which are restrictive to most life. In 2007 tardigrades were shown to survive and reproduce normally during an 11-day low Earth orbit on the Foton-M3 Capsule. We speculate that mechanisms tardigrades have evolved to withstand extreme environments on Earth may, as a side-effect, confer protection against the stresses of spaceflight. This makes tardigrades a uniquely valuable system for studying responses to spaceflight. We have sequenced the genome of the tardigrades Hypsibius dujardini, as well as developed and validated experimental and computational approaches for measuring the effect of different environmental conditions on tardigrade gene expression – allowing us to identify mechanisms used by tardigrades to protect themselves from different stresses. We have also developed a reverse genetic approach, RNA interference, for tardigrades that allows us to directly investigate the role of a gene in conferring tolerance to an environment. We will use these approaches to study tardigrades’ initial, as well as multigenerational, response to spaceflight and use RNA interference to test the functionality of the genes identified in our study. Next-generation transcriptome sequencing will be conducted on tardigrades cultures kept 0 generations (founding generation) and 4 generations onboard the International Space Station (ISS). Differential expression analysis will be conducted to compare ISS spaceflight timepoints, ground controls, and tardigrades exposed to other extreme stresses (e.g., desiccation, freezing). This approach will allow us to identify potential mediators of stress tolerance, which will serve as candidates for functional RNA interference experiments. Understanding how tardigrades tolerate spaceflight will better guide future research into countermeasures and therapies for humans exposed to the stresses of prolonged space travel. This proposal's strengths are: the use of an organism that is suited to studying mechanisms of multigenerational tolerance of extreme environments and that has an established RNA interference method for confirming the function of genes identified in our study, our Preliminary Results that validate our proposed approach and technical capabilities as well as the uniqueness and suitability of tardigrades that will allow us to conduct this study. The participants for this study are comprised of experts in tardigrades' stress response and have considerable experience with next-generation sequencing and analysis of non-model organisms. The proposed experiments directly address recommendation AH16 of the Decadal Survey and are in line with recommendation OCB-5 and CMM-5 of NASA’s Multigenerational and Developmental Biology of Invertebrates Research Emphasis as well as NASA’s Fundamental Space Biology Plan 2010-2020 goals. Completion of our proposal will identify genes required for tardigrades to survive multigenerational spaceflight and will be a key step towards developing countermeasures and therapies for stresses associated with prolonged human exposure to space environments.

Research Impact/Earth Benefits: Along with using mechanisms of stress tolerance to counteract detrimental effects of space travel, data from our proposed experiments could be used in the long term toward solving serious problems in the field of human health. Utilizing mechanisms that allow tardigrades to stabilize their cellular proteins and nucleic acids has been proposed as an option for the dry storage and stabilization of vaccines and other biomaterials (Guo et al., 2000; Wolkers et al., 2001; Puhlev et al., 2001). Because current techniques for vaccine production, distribution, and storage nearly always require a constant cold chain (e.g., -80 and 20 degrees C freezers), these processes are extremely expensive. Some estimates put cold chain costs at around 80% of the total cost of vaccination (Chen et al., 2011). By generating additional stress response datasets, such as response to microgravity, freezing, irradiation, and hypoxia, we will increase our ability and that of other researchers to identify specific mediators of desiccation tolerance, which will then be applied to this and similar problems.

Additionally, a better understanding of mechanisms of stress tolerance could lead to the development of drought and/or freeze tolerant crops.

Guo, N., Puhlev, I., Brown, D. R., Mansbridge, J., & Levine, F. (2000). Trehalose expression confers desiccation tolerance on human cells. Nature biotechnology, 18(2), 168-171.

Wolkers, W. F., Walker, N. J., Tablin, F., & Crowe, J. H. (2001). Human platelets loaded with trehalose survive freeze-drying. Cryobiology, 42(2), 79-87.

Puhlev, I., Guo, N., Brown, D. R., & Levine, F. (2001). Desiccation tolerance in human cells. Cryobiology, 42(3), 207-217.

Chen, X. et al. (2011). Improving the reach of vaccines to low-resource regions, with a needle-free vaccine delivery device and long-term thermostabilization. J. Controlled Release 152, 349–355.

Task Progress & Bibliography Information FY2020 
Task Progress: New project for FY2020.

NOTE this continues "Using Water Bears to Identify Biological Countermeasures to Stress During Multigenerational Spaceflight," grant NNX15AB44G, due to Principal Investigator Boothby's move to University of Wyoming from University of North Carolina. See that project for previous reporting.

Bibliography Type: Description: (Last Updated: 07/24/2020)  Show Cumulative Bibliography Listing
 None in FY 2020