We have made significant progress over the past 10 months preparing IRB applications and receiving approvals from the UCSD and NASA-JSC Institutional Review Boards (IRBs). Moreover, we also made significant progress with optimizing and scheduling our pre- and post-flight tests to maximize their scientific value and to minimize impacts and risks to ISS crew members. After receiving official notice on 7 April 2010 from the Human Research Program at NASA JSC that our proposal was selected as a “Flight Definition” project, we submitted an IRB application to UCSD. We received official approval for our IRB application from UCSD on 27 July 2010, back-dated to our “pending approval” date of 24 June 2010.
Next, we submitted our approved UCSD protocol to the NASA-JSC CPHS on 29 July 2010. The NASA-JSC CPHS tabled their full review of our UCSD-approved protocol until their NASA Radiation Isotopes Subcommittee reviewed and approved it. After we were contacted in late August 2010 to provide more information on radiation dosages and to reduce dosages to levels as low as reasonably achievable, the NASA Radiation Isotopes Subcommittee met on 15 September 2010 to reconsider our protocol. After consideration of the reduced dosages and the impact upon astronaut career limits, the NASA Radiation Isotopes Subcommittee approved the proposal with the specification that the Consent Form be revised and individualized to provide astronauts with both career limit impact and health risks of combined space flight and scientific investigation induced-radiation exposures. Prior to an ISS Investigator Working Group meeting on 22 November 2010, the PI received feedback from Drs. Jan Meck and Scott Parazynski that the Astronaut Office thought that the radiation levels were too high and crew consent would be difficult to obtain. Thus, during a meeting of two Co-Is (Drs. Lotz and Chiang) and myself, we decided to delete the post-pre-flight PET test using Na-F and make all PET tests optional for crew members as follows:
1) Post-flight FDG of the whole spine only (10 mSv/0.42 mSv/day=24 extra days on ISS)
2) Pre- and Post-flight FDG scans of the whole spine (48 extra days on ISS)
3) Post-flight Na-F if abnormal post-flight MRI (7.5mSv/0.42mSv/day= 18 extra days on ISS)
4) No PET scans- minimal radiation from fluoroscopy only (3.4 mSv or 8 extra days on ISS)
These four options would allow crew members to decide what radiation dosage best suits their career limits and helps them manage their individual risks.
Also, following the ISS Investigator Working Group meeting on 22 November 2010, we moved our MRI and MRS testing site to UTMB Victory Lakes to reduce ISS crew transport times and evaluated the standard, back strength tests that are performed using the NASA JSC Biodex equipment. We decided that Biodex data will be of value to us, but we also need a form of endurance test in spine posture as the isometric endurance is more functional outcome for spinal fatigue. Below is a list of our action items from the ISS Investigator Working Group meeting on 22 November 2010, followed by our responses: 1. For IVD, SPRINT, ICV: a. Gather all MRI requirements. See attached NASA IRB application to be submitted tomorrow. b. Determine if Victory Lakes can accommodate all MRI requirements. Yes, we think so depending on NASA’s negotiations with UTMB for use of their facilities. c. Determine to what degree, if any, MRI sessions can be combined. Not possible as far as we know as we request R+1 imaging. d. Determine washout time for tracers (how long between IVD and ICV MRI’s). No washout time.
2. For FTT, SPRINT and IVD: a. Can any muscle function/strength measurements from SPRINT/FTT be shared with IVD? No, but we are checking is CSE tests are sufficient for our purposes.
After responding to the NASA-JSC CPHS, we received official approval of our revised NASA protocol and consent form on 7 February 2011.
Before and after this approval by the NASA-JSC CPHS, we have been coordinating and optimizing our protocol and pre- and post-flight crew-testing schedule with several personnel from the Biomedical Research and Countermeasures Projects Branch and the Bioastronautics Team of the International Space Station Medical Project at NASA JSC.
On 12 April 2011, Sayson and Hargens presented a review paper “Back Pain Mechanisms in Space” at the IAA Humans in Space meeting in Houston, TX: J.V. Sayson, A.R. Hargens. Back Pain Mechanisms in Space. IAA 18th Humans in Space Symposium: “Integration and Cooperation in the Next Golden Age of Human Space Flight.” April 11-15, 2011.
On 14 April 2011, also at the IAA 18th Humans in Space Symposium, our team presented an abstract “STS-131: Effects of Microgravity on the Creep Behavior of Murine Intervertebral Discs.” J.F. Bailey, K.K. Cheng, A.R. Hargens, K. Masuda, and J.C. Lotz. STS-131: Effects of Microgravity on the Creep Behavior of Murine Intervertebral Discs. IAA 18th Humans in Space Symposium: “Integration and Cooperation in the Next Golden Age of Human Space Flight.” April 11-15, 2011.
Following this meeting, we performed pilot studies of our upright MRI, spinal kinematics, MRI and MRS tests at facilities nearby NASA JSC. The times for each test were within the limits which we cited in our IRB applications; and the tests were valuable in order to optimize tests on actual crew members planned for next year. We plan to conduct another pilot test in San Diego 19 May 2011 in order to optimize further the back pack design and stability conditions for our upright MRI tests. Related to the tests of spinal kinematics, we noted an upright L5/S1 motion curve with three peaks in one of our investigators who served as a control subject. Our expert, Adam Deitz, compared the results multiple times with the raw images and confirmed that the graph was true to the motion in the video. We found that our control subject is highly mobile, especially in the upper lumbar region. Based on the recumbent flexion/extension data, it appears that his L5/S1 is the least stiff of all of his spinal segments, and that L3/L4 and L4/L5 are the most-stiff segments. These pilot studies demonstrate that dynamic fluoroscopy is a very sensitive tool for quantifying lumbar motion and should provide valuable information as to how long-duration space flight alters spinal movement patterns.
Also, at the IAA Humans in Space meeting in Houston, our team was informed that our proposed PET/CT tests of spinal inflammation and injury were deleted because they were deemed to be insufficiently valuable from a benefit/risk ratio. We hope to receive a formal letter from NASA explaining this decision.
We are finalizing a review paper “Back Pain in Space and Post-Flight Spine Injury: Mechanisms and Countermeasure Development” by J Sayson, J Lotz, S Parazynski and A Hargens for submission to Acta Astronautica within the next month.
The next few months will include revising our UCSD and NASA IRB applications to incorporate changes related to the PET/CT scans and other minor adjustments which were derived from our pilot studies in April 2011.
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