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Project Title:  Feasibility of Synthetic Biology Countermeasures for Human Exploration Beyond Low Earth Orbit Reduce
Images: icon  Fiscal Year: FY 2022 
Division: Space Biology 
Research Discipline/Element:
Space Biology: Microbiology  
Start Date: 12/01/2021  
End Date: 11/30/2023  
Task Last Updated: 01/19/2022 
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Principal Investigator/Affiliation:   Settles, Andrew  Ph.D. / NASA Ames Research Center 
Address:  AST Life Support Studies 
 
Moffett Field , CA 94035-1000 
Email: andrew.m.settles@nasa.gov 
Phone: 352-283-2767  
Congressional District: 18 
Web:  
Organization Type: NASA CENTER 
Organization Name: NASA Ames Research Center 
Joint Agency:  
Comments:  
Co-Investigator(s)
Affiliation: 
Hindupur, Aditya  Ph.D. KBR/NASA Ames Research Center 
Project Information: Grant/Contract No. Internal Project 
Responsible Center: NASA ARC 
Grant Monitor: Loftus, David  
Center Contact: 650-604-1011 
david.j.loftus@nasa.gov 
Solicitation / Funding Source: 2021 Space Biology NNH21ZDA001N-LEIA E.10. Lunar Explorer Instrument for Space Biology Applications 
Grant/Contract No.: Internal Project 
Project Type: GROUND 
Flight Program:  
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Space Biology Element: (1) Microbiology
Space Biology Cross-Element Discipline: None
Space Biology Special Category: None
Flight Assignment/Project Notes: NOTE: Project dates (POP) changed; now 12/1/2021-11/30/2023 per F. Hernandez/ARC (Ed., 1/19/22)

Task Description: Microbial production of bioactive compounds, such as vitamins or pharmaceuticals, can reduce risks for deep-space crewed missions. Yeast are excellent chassis organisms to express countermeasure products due to their long shelf-life viability. Yeast have robust synthetic biology technology to transfer whole biosynthesis pathways for synthesis of desired products. NASA has invested in yeast production of micronutrients that are known to have a short shelf-life in prepackaged foods, with demonstrated success on the International Space Station. However, low-Earth orbit does not test yeast for resistance to higher radiation levels and the more extreme environment of deep space.

The goal of this proposal is to develop methods to preserve, grow, and measure production of desired synthetic biology products from edible yeast, using the BioSensor platform. BioSensor automates yeast culture activation and monitors growth with light absorbance of specific wavelengths produced by light emitting diodes (LED). The project goal is to expand the capability of BioSensor to enable monitoring synthetic biology traits along with cell growth and metabolism. The central objectives are: 1) Develop methods to predict synthetic biology production traits, namely carotenoids and recombinant proteins, using multivariate statistical models based on three wavelength light absorbance. We anticipate that the BioSensor platform will need to be modified to replace one of the current wavelengths to a blue LED; 2) Yeast may be overly sensitive to deep space radiation, and we will engineer carotenoid producing strains to express a DNA damage protection protein from tardigrades; 3) Non-conventional, yeast species may be more efficient for recombinant protein expression in deep space conditions. We will engineer three edible species to produce a target protein that absorbs blue light, to enable monitoring of recombinant protein and carotenoids in the same BioSensor device; and 4) Determine strain and media storage conditions as well as test the multiwavelength light monitoring strategy to establish the requirements and methodology for a future lunar surface mission.

The project is expected to advance the remote sensing technology in the BioSensor platform. A future flight experiment is expected to develop fundamental knowledge on the effects of deep space on protein expression and metabolite production. In addition, the project directly addresses critical gaps to advance crewed missions in deep space exploration. The specific carotenoids, beta-carotene and zeaxanthin, are important micronutrients to prevent macular degeneration and have been identified as potential countermeasures to maintain vision in astronauts during deep space missions. Recombinant protein expression in non-conventional yeast will demonstrate feasibility for production of bioactive protein therapies in deep space missions. These synthetic biology approaches are critical to provide products that are sensitive to radiation and have short shelf lives in prepackaged foods and medicines.

Research Impact/Earth Benefits:

Task Progress & Bibliography Information FY2022 
Task Progress: New project for FY2022.

Bibliography Type: Description: (Last Updated: ) 

Show Cumulative Bibliography Listing
 
 None in FY 2022