Task Progress:
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This project addresses the topic: “Lab Analysis Point-of-Care Device Evaluation and Downselect” of the Human Research Roadmap (HRR) gap Osteo 5 that states, “we need an in-flight capability to monitor bone turnover and bone mass changes during spaceflight.” One bone remodeling biomarker that can be directly correlated with bone resorption from a non-invasive urine specimen is NTx, a degradation by-product of type I collagen. NTx levels in urine were recently used to evaluate bone health during the NASA Twin Study and is a biomarker of interest, referred to in HRR’s Osteo 5 gap. To address this HRR gap, we have developed a methodology using a printed electrochemical sensor capable of detecting NTx in urine. Our goal is to create a highly adaptable and versatile approach that utilizes fabrication processes consistent with in-space manufacturing, thus enabling the manufacture of point-of-care devices during flight. Going forward, we plan to expand the sensor’s capability to measure a variety of bone remodeling biomarkers simultaneously. However, NTx serves as our initial target for this project and as a proof-of-concept for our approach.
The project objective is to create a space-suitable, printed sensor strip for quantifiable NTx detection coupled to a urine collection device. To accomplish this objective, we have focused on the following Aims. Aim 1: Develop conductive, telopeptide selective and dielectric inks for printed sensor. Aim 2: Print and electrochemically characterize 3-electrode device for NTx detection. Aim 3: Integrate sensor with urine collection device and handheld potentiostat hardware.
During this project, we have created a hands-free and robust manufacturing process using microdispense, inkjet, and 3D printing to generate an electrochemical sensor for measuring NTx from a urine specimen. The sensor utilizes a carbon nanotube ink, which improves the electrochemical response and gold nanoparticles to provide selective surfaces for NTx detection. Using those optimized and pre-screened electronic inks, 3-electrode electrochemical sensors have been printed using inkjet and precision microdispense instrumentation. Both devices were characterized for their electrochemical performance and robustness. Fluid delivery based on lateral flow materials was designed to carry the urine specimen to the sensor surface. Finally, sensors were packaged into reusable cassette holders and interfaced with a handheld reader for simple and reliable operation.
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Abstracts for Journals and Proceedings
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Koehne, J. E., Cordeiro, M. "Printed electrochemical sensor for quantifying bone density loss in microgravity." 2021 NASA Human Research Program Invesitgators' Workshop, virtual, February 1-4, 2021. Abstracts. 2021 NASA Human Research Program Investigators' Workshop, virtual, February 1-4, 2021. , Feb-2021
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Abstracts for Journals and Proceedings
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Koehne JE. "Printed flexible sensors for NASA applications." 4DMS + SoRo Summer School, virtual, June 21-22, 2021. Abstracts. 4DMS + SoRo Summer School, virtual, June 21-22, 2021. , Jun-2021
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Abstracts for Journals and Proceedings
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Koehne JE. "In-space manufacturing of point-of-care diagnostic devices." American Chemical Society CME NASA STEM Symposium, Atlanta, Georgia, August 23-24, 2021. Abstracts. American Chemical Society CME NASA STEM Symposium, Atlanta, Georgia, August 23-24, 2021. , Aug-2021
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Abstracts for Journals and Proceedings
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Koehne JE. "Printed sensors and electronics for sustained human spaceflight." First International Conference on Smart Green Connected Societies, virtual, November 29-30, 2021. Abstracts. First International Conference on Smart Green Connected Societies, virtual, November 29-30, 2021. , Nov-2021
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Abstracts for Journals and Proceedings
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Koehne JE. "In-space manufacturing to support human spaceflight. " Materials Research Society, Boston, Massachusetts, November 28-December 2, 2021. Abstracts. Materials Research Society, Boston, Massachusetts, November 28-December 2, 2021. , Nov-2021
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Abstracts for Journals and Proceedings
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Koehne JE. "Carbon nanomaterial based electrochemical sensors and devices for NASA missions." IEEE NMDC, Vancouver, Canada, December 12-15, 2021. Abstracts. IEEE NMDC, Vancouver, Canada, December 12-15, 2021. , Dec-2021
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Abstracts for Journals and Proceedings
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Cordeiro M, Espinoza C, Koehne JE. "Printed electrochemical sensor for quantifying bone density loss in microgravity." 2022 NASA Human Research Program Investigators' Workshop, virtual, February 8, 2022. Abstracts. 2022 NASA Human Research Program Investigators' Workshop, virtual, February 8, 2022. , Feb-2022
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Abstracts for Journals and Proceedings
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Cordeiro M. "Printed wearable electrochemical sensor for monitoring human performance markers during human spaceflight." Electrochemical Society Meeting, Vancouver, Canada, May 31, 2022. Abstracts. Electrochemical Society Meeting, Vancouver, Canada, May 31, 2022. , May-2022
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Abstracts for Journals and Proceedings
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Koehne JE. "Bioanalytical sensors for support human exploration missions." Gordon Research Conference on Bioanalytical Sensors, Newport, Rhode Island, June 26, 2022. Abstracts. Gordon Research Conference on Bioanalytical Sensors, Newport, Rhode Island, June 26, 2022. , Jun-2022
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Articles in Other Journals or Periodicals
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Cordeiro M, Koehne JE. "Fully inkjet printed carbon nanotube electrochemical sensor for NASA missions." ECS Sensors Plus, submitted. , Jan-2023
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