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Project Title:  Bion-M2 Spaceflight-Induced Effects on Neurovascular Remodeling and Blood-Retina Barrier Function: Role of Oxidative Stress Reduce
Images: icon  Fiscal Year: FY 2020 
Division: Space Biology 
Research Discipline/Element:
Space Biology: Cell & Molecular Biology   | Animal Biology: Vertebrate  
Start Date: 05/01/2020  
End Date: 04/30/2023  
Task Last Updated: 06/30/2020 
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Principal Investigator/Affiliation:   Mao, Xiao Wen  M.D. / Loma Linda University 
Address:  Radiation Medicine 
11175 Campus Street, Rm A1010, Chan Shun Pavilion 
Loma Linda , CA 92350-0001 
Email: xmao@llu.edu 
Phone: 909-558-8373  
Congressional District: 31 
Web:  
Organization Type: UNIVERSITY 
Organization Name: Loma Linda University 
Joint Agency:  
Comments:  
Co-Investigator(s)
Affiliation: 
Delp, Michael  Ph.D. Florida State University 
Pecaut, Michael  Ph.D. Loma Linda University 
Sun, Shu-Wei  Ph.D. Loma Linda University 
Wang, Charles  M.D., Ph.D. Loma Linda University 
Project Information: Grant/Contract No. 80NSSC20K0986 
Responsible Center: NASA ARC 
Grant Monitor: Griko, Yuri  
Center Contact: 650-604-0519 
Yuri.V.Griko@nasa.gov 
Solicitation: 2018 Space Biology NNH18ZTT002N:Russian Bion-M2 Mission 
Grant/Contract No.: 80NSSC20K0986 
Project Type: FLIGHT 
Flight Program: Bion-M2 
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Space Biology Element: (1) Cell & Molecular Biology
(2) Animal Biology: Vertebrate
Space Biology Cross-Element Discipline: None
Space Biology Special Category: None
Task Description: The health risk of spaceflight-induced neuronal damage and potential adverse neurovascular effects has long been a concern. A recent report shows that more than 50% of the astronauts returning from space were diagnosed with visual problems that can cause blurry vision. Our previous studies from mice that had been subjected to spaceflights (space shuttle mission Space Transportation System (STS)-118 and STS-135) showed that environmental conditions during space travel lead to oxidative stress and induce adverse microvessel remodeling in the retina. To date, the mechanisms behind these effects are not fully understood.

The objective of this proposed project is to characterize the effect of Bion-M2 mission on retinal vascular remodeling and visual function. Furthermore, the molecular and cellular mechanisms involving oxidative stress-induced vascular response and impaired blood-retina-barrier (BRB) and blood-brain barrier (BBB) integrity will be investigated.

Mature male and female mice will be exposed to spaceflight environment on board Bion-M2 mission for 30 days and compared to that of ground-based control groups. Animals will be sacrificed 3-15 days after return to Earth. Non-invasive intraocular pressure (IOP) and electroretinography (ERG) will be used to measure intraocular pressure and retinal function before sacrifice. Eyes and brains will be removed for fixed or frozen for ex vivo diffusion tensor imaging (DTI) imaging, genomic profiling, and immunohistological analysis.

Together, our unique, integrative, quantitative approaches with advanced imaging techniques and comprehensive genomic analysis will provide insight into the cellular mechanism of spaceflight-induced effects on the interaction of parenchymal activity with neurovascular response and provide criteria for risks of functional detriments. Understanding how spaceflight impacts neurovascular remodeling and BRB/BBB function will help focus the approach for more effective countermeasures during human spaceflight and planetary exploration.

Research Impact/Earth Benefits:

Task Progress & Bibliography Information FY2020 
Task Progress: New project for FY2020.

Bibliography Type: Description: (Last Updated: 08/03/2020)  Show Cumulative Bibliography Listing
 
 None in FY 2020