Task Progress:
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Following the COVID-19 pandemic and laboratory shutdown, in vivo experiments have been delayed in 2020. During the first semester (July 2020-December 2020), we worked towards completion of the Aim 1, by assessing how males adjust to partial reloading at 20, 40, and 100% of weight-bearing following an extensive time spent in a microgravity analogue, and thus disused.
As our goal was to assess the existence of a “reloading injury” following disuse, as would be the case when astronauts would land on the Moon, Mars, or Earth following a trip in space, animals were re-ambulated for either 4 or 24 hours and monitored. We did not encounter methodological problems during this experiment and successfully completed all groups by the end of 2020.
Separately, we assessed the impact of disuse on female rats compared to normally-loaded controls, and analyzed all results both in vivo and ex vivo. Therefore, we were able to compare if males and females respond similarly to disuse-induced muscular deconditioning, and submitted a manuscript reflecting our findings in 2021. The manuscript is currently under revision.
In the second semester (January 2021-June 2021), we focused on “reloading injury” following disuse in female animals (Aim 2). We used a paradigm strictly identical to what has been done previously with male rats, and successfully completed the 4 and 24 hours groups for females.
Ex vivo analyses have been completed and a manuscript recapitulating the neuromuscular changes in both sexes is currently in preparation, and should be submitted for publication shortly.
Moreover, we explored the potential relationship between estrous cycle maintenance and musculoskeletal functions using both animals from our current project and previous results. The findings have been summarized in a manuscript that has been submitted for publication in 2021.
Overall, our work this year has aimed to highlight how sex influences animals adaptation to musculoskeletal disuse, and if partial reloading significantly impacts musculoskeletal health.
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Abstracts for Journals and Proceedings
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Rosa-Caldwell ME, Mortreux M, Sung DM, Schreurs AS, Bouxsein ML, Rutkove SB. "Low testosterone status differentially affects musculoskeletal outcomes after exposure to micro- or partial gravity." 36th Annual Meeting of the American Society for Gravitational and Space Research, Virtual Meeting, November 5-6, 2020. Abstracts. 36th Annual Meeting of the American Society for Gravitational and Space Research, Virtual Meeting, November 5-6, 2020. , Nov-2020
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Abstracts for Journals and Proceedings
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Mortreux M, Rosa-Caldwell ME, Sung DM, Stiehl ID, Rutkove SB. "Early adaptation to increased mechanical loading after disuse: muscular response in male and female rats." 36th Annual Meeting of the American Society for Gravitational and Space Research, Virtual Meeting, November 5-6, 2020. Abstracts. 36th Annual Meeting of the American Society for Gravitational and Space Research, Virtual Meeting, November 5-6, 2020. , Nov-2020
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Articles in Peer-reviewed Journals
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Rosa-Caldwell ME, Mortreux M, Kaiser UB, Sung DM, Bouxsein ML, Dunlap KR, Greene NP, Rutkove SB. "The oestrous cycle and skeletal muscle atrophy: Investigations in rodent models of muscle loss." Exp Physiol. 2021 Sep 26. https://doi.org/10.1113/EP089962 ; PMID: 34569104; PMCID: PMC8639792
, Sep-2021
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Articles in Peer-reviewed Journals
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Mortreux M, Rosa-Caldwell ME, Stiehl ID, Sung DM, Thomas NT, Fry CS, Rutkove SB. "Hindlimb suspension in Wistar rats: Sex-based differences in muscle response." Physiol Rep. 2021 Oct;9(19):e15042. https://doi.org/10.14814/phy2.15042 ; PMID: 34612585; PMCID: PMC8493566
, Oct-2021
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Articles in Peer-reviewed Journals
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Mortreux M, Rosa-Caldwell ME. "Approaching gravity as a continuum using the rat partial weight-bearing model. " Life. 2020 Oct 8;10(10):235. Review. https://doi.org/10.3390/life10100235 ; PMID: 33049988; PMCID: PMC7599661
, Oct-2020
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Articles in Peer-reviewed Journals
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Malkani S, Chin CR, Cekanaviciute E, Mortreux M, Okinula H, Tarbier M, Schreurs AS, Shirazi-Fard Y, Tahimic CGT, Rodriguez DN, Sexton BS, Butler D, Verma A, Bezdan D, Durmaz C, MacKay M, Melnick A, Meydan C, Li S, Garrett-Bakelman F, Fromm B, Afshinnekoo E, Langhorst BW, Dimalanta ET, Cheng-Campbell M, Blaber E, Schisler JC, Vanderburg C, Friedländer MR, McDonald JT, Costes SV, Rutkove S, Grabham P, Mason CE, Beheshti A. "Circulating miRNA spaceflight signature reveals targets for countermeasure development." Cell Rep. 2020 Dec 8;33(10):108448. https://doi.org/10.1016/j.celrep.2020.108448 ; PMID: 33242410
, Dec-2020
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Articles in Peer-reviewed Journals
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Willey JS, Britten RA, Blaber E, Tahimic CGT, Chancellor J, Mortreux M, Sanford LD, Kubik AJ, Delp MD, Mao XW. "The individual and combined effects of spaceflight radiation and microgravity on biologic systems and functional outcomes." J Environ Sci Health C Toxicol Carcinog. 2021 Apr 27;39(2):129-79. https://doi.org/10.1080/26896583.2021.1885283 ; PMID: 33902391; PMCID: PMC8274610
, Apr-2021
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