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Project Title:  Impact of Radiation Exposure on a 3D In Vitro Model of Human Bone Marrow Reduce
Fiscal Year: FY 2020 
Division: Human Research 
Research Discipline/Element:
TRISH--TRISH 
Start Date: 06/01/2019  
End Date: 08/31/2020  
Task Last Updated: 01/04/2021 
Download report in PDF pdf
Principal Investigator/Affiliation:   George, Steven  Ph.D. / University of California, Davis 
Address:  1 Shields Ave, Biomedical Engineering 
 
Davis , CA 95616 
Email: scgeorge@ucdavis.edu 
Phone: 530-752-9978  
Congressional District:
Web:  
Organization Type: UNIVERSITY 
Organization Name: University of California, Davis 
Joint Agency:  
Comments:  
Co-Investigator(s)
Affiliation: 
Prebys, Eric  Ph.D. University of California, Davis 
Project Information: Grant/Contract No. NNX16AO69A-FIP0016 
Responsible Center: TRISH 
Grant Monitor:  
Center Contact:   
Solicitation / Funding Source: TRISH--Focused Investigations 
Grant/Contract No.: NNX16AO69A-FIP0016 
Project Type: GROUND 
Flight Program:  
TechPort: Yes 
No. of Post Docs:
No. of PhD Candidates:
No. of Master's Candidates:
No. of Bachelor's Candidates:
No. of PhD Degrees:
No. of Master's Degrees:
No. of Bachelor's Degrees:
Human Research Program Elements: None
Human Research Program Risks: None
Human Research Program Gaps: None
Task Description: Focused Investigation Project

The primary goal of this project is to characterize the impact of acute ionizing radiation (at levels that mimic deep space exploration) on the health and function of a human bone marrow using a microphysiological system model of human marrow (“bone marrow-on-a-chip” or BMoaC). The high significance of this project is derived from the unique and potentially dangerous levels of ionizing radiation exposure for astronauts on deep space missions, and the highly radio-sensitive features of human bone marrow, in particular the hematopoietic stem cell. Recent reports in simple monolayer culture systems suggest that both the hematopoietic stem cells (HSCs) and the supporting stromal cells (e.g., mesenchymal stem cell, MSC) are acutely effected by ionizing radiation which not only disrupts hematopoiesis, but also increases the incidence of leukemias.

Specific Aim: Determine a dose-response curve between ionizing radiation (proton) and normal biological function of human bone marrow (leukocyte production) using a microphysiological system model of human bone marrow.

Research Impact/Earth Benefits: The potential impact of the research lies in the use of a human bone marrow model, and the demonstration that the production and release of CD15+ (neutrophils) cells may be quite sensitive to doses that exceed 1 Gy. This is relevant as 1 Gy is the anticipated dose to astronauts on a mission to Mars and back.

Task Progress & Bibliography Information FY2020 
Task Progress: The primary goal of this project is to characterize the impact of acute ionizing radiation (at levels that mimic deep space exploration) on the health and function of a human bone marrow using a microphysiological system model of human marrow (bone marrow-on-a-chip or BMoaC). The high significance of this project is derived from the unique and potentially dangerous levels of ionizing radiation exposure for astronauts on deep space missions, and the highly radio-sensitive features of human bone marrow, in particular the hematopoietic stem cell. Recent reports in simple monolayer culture systems suggest that both the hematopoietic stem cells (HSC) and the supporting stromal cells (e.g., mesenchymal stem cell, MSC) are acutely effected by ionizing radiation which not only disrupts hematopoiesis, but also increases the incidence of leukemias.

Specific Aim: Determine a dose-response curve between ionizing radiation (proton) and normal biological function of human bone marrow (leukocyte production) using a microphysiological system model of human bone marrow.

Key Finding: We were able to demonstrate a dose-dependent decrease in CD45 and CD15 cells in the bone marrow over a dose range of 0.1 - 10 Gy. In addition, we observed a step change (decrease) in the release of CD15+ cells from the device for radiation doses exceeding 1.0 Gy.

Bibliography Type: Description: (Last Updated: )  Show Cumulative Bibliography Listing
 
 None in FY 2020
Project Title:  Impact of Radiation Exposure on a 3D In Vitro Model of Human Bone Marrow Reduce
Fiscal Year: FY 2019 
Division: Human Research 
Research Discipline/Element:
TRISH--TRISH 
Start Date: 06/01/2019  
End Date: 05/31/2020  
Task Last Updated: 06/10/2019 
Download report in PDF pdf
Principal Investigator/Affiliation:   George, Steven  Ph.D. / University of California, Davis 
Address:  1 Shields Ave, Biomedical Engineering 
 
Davis , CA 95616 
Email: scgeorge@ucdavis.edu 
Phone: 530-752-9978  
Congressional District:
Web:  
Organization Type: UNIVERSITY 
Organization Name: University of California, Davis 
Joint Agency:  
Comments:  
Project Information: Grant/Contract No. NNX16AO69A-FIP0016 
Responsible Center: TRISH 
Grant Monitor:  
Center Contact:   
Solicitation / Funding Source: TRISH--Focused Investigations 
Grant/Contract No.: NNX16AO69A-FIP0016 
Project Type: GROUND 
Flight Program:  
TechPort: Yes 
No. of Post Docs:  
No. of PhD Candidates:  
No. of Master's Candidates:  
No. of Bachelor's Candidates:  
No. of PhD Degrees:  
No. of Master's Degrees:  
No. of Bachelor's Degrees:  
Human Research Program Elements: None
Human Research Program Risks: None
Human Research Program Gaps: None
Task Description: Focused Investigation Project

The primary goal of this project is to characterize the impact of acute ionizing radiation (at levels that mimic deep space exploration) on the health and function of a human bone marrow using a microphysiological system model of human marrow (“bone marrow-on-a-chip” or BMoaC). The high significance of this project is derived from the unique and potentially dangerous levels of ionizing radiation exposure for astronauts on deep space missions, and the highly radio-sensitive features of human bone marrow, in particular the hematopoietic stem cell. Recent reports in simple monolayer culture systems suggest that both the hematopoietic stem cells (HSCs) and the supporting stromal cells (e.g., mesenchymal stem cell, MSC) are acutely effected by ionizing radiation which not only disrupts hematopoiesis, but also increases the incidence of leukemias.

Specific Aim: Determine a dose-response curve between ionizing radiation (proton) and normal biological function of human bone marrow (leukocyte production) using a microphysiological system model of human bone marrow.

Research Impact/Earth Benefits:

Task Progress & Bibliography Information FY2019 
Task Progress: New project for FY2019.

Bibliography Type: Description: (Last Updated: )  Show Cumulative Bibliography Listing
 
 None in FY 2019