NOTE: End date changed to 7/31/2021 per E. Urquieta/TRISH (Ed., 4/28/21)
NOTE: End date changed to 10/31/2019 per E. Urquieta/TRISH (Ed., 5/23/19)
This project aims to evaluate the potential impact of the space flight environment on the regulation of molecular pathways mediating cellular stress responses. We performed a first-of-its-kind pilot feasibility study to assess space flight-associated changes in exosomes derived from peripheral blood (PB) plasma collected 10 days before the launch (L -10) and the day of landing (R 0) from two astronauts who participated in STS-100 and STS-104 missions. Our preliminary pilot experiment results suggest that spaceflights may induce remarkable changes in the cargo of circulating plasma exosomes that may reflect alterations in multiple gene and protein pathways in various tissues and cells. This work represents a pilot/feasibility study to identify plasma exosomal miRNA as a source of blood derived epigenetic biomarker identification.