Task Progress:
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Two Specific Aims were proposed to support the microvascular hypothesis that addresses NASA solicitation requirements for an 'accelerated, new scientific approach to produce novel scientific knowledge and deliver initial proof-of-concept mappings.' The proposal further addressed HRP Risk ‘Microgravity-induced Visual Alterations and Intracranial Pressure’ by investigation of the HRP Gap ‘VIIP1 What is the etiology of visual acuity and ocular structural and functional changes in-flight and post-flight?’ ('VIIP' is now designated as Spaceflight Associated Neuro-ocular Syndrome (SANS)). The study further addressed the first objective of the NASA solicitation, quantification of crew health and performance risks. Below are the aims proposed for the study. The second aim was accepted by NASA, resulting in expansion of the original study design.
Aim 1—Alterations in vascular patterning of the human retina responding to fluid shifts incurred by long-duration head-down tilt bed rest will be mapped and quantified.
Aim 2—Alterations in the retinal vascular patterning of astronauts before and after spaceflight will be mapped and quantified, should NASA decide to provide additional resources to support this Aim.
Work proposed for the study is now complete, including insightful biostatistical and ophthalmic correlations. Arterial and venous branching were analyzed by VESGEN in the retinas of eight Crew Members before and after six-month flight to the ISS, and of six subjects before and after 70-day 6° HDT Bed Rest. Trends in pre to post status within the retinas of Crew Members and HDT subjects measured by the VESGEN analysis are opposite. By two confirming measures of vascular branching complexity, the fractal dimension (Df) and length density of small vessels (Lv=5), the space-filling capacity of arterial and venous trees, decreased for a majority of Crew Members. In contrast, vascular densities increased by these same parameters for a majority of HDT subjects. As predicted, the length density of larger vessels (Lv1-4) remained relatively constant. The assignment of vascular branching generations into large and small vessels by VESGEN further confirmed that vascular adaptations to microgravity and HDT occurred primarily at the level of the smaller arteries and veins.
One of 16 Crew Member retinas was found by NASA to display early SANS using a clinical optic disc edema (ODE) measure. A subclinical vascular pathology index (SVPI) was therefore calculated to compare the magnitude of vascular change measured by VESGEN in the SANS retina with the 15 other retinas assessed as clinically normal by clinical ocular measures that include ODE and total retinal and choroidal thicknesses. By the SVPI, twelve retinas displayed vascular decreases from 6% to 76%, compared to the SANS retina. Relationships of vascular change to other parameters such as globe flattening and total retinal and choroidal thickness were investigated as mixed effects. For example, vascular decreases were associated with increased total retinal and retinal nerve fiber layer thicknesses.
Results of this first proof-of-concept VESGEN study support further investigation of vascular patterning as a potential biomarker of early vascular changes related to SANS etiology and susceptibility.
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Abstracts for Journals and Proceedings
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Murray MC, Vizzeri GM, Taibbi G, Mason SS, Young M, Zanello SB, Parsons-Wingerter P. "Differences in Pre and Post Vascular Patterning within Retinas of ISS Crew Members and Head-Down Tilt Subjects by VESGEN Analysis." Oral presentation at Spaceflight-Associated Neuro-Ocular Syndrome (SANS/VIIP) – Flight Findings, Human Research Program Investigators’ Workshop (IWS), 'The Gateway to Mars,' 2018 Human Research Program Investigators' Workshop, Galveston, TX, January 22-25, 2018. 2018 Human Research Program Investigators' Workshop, Galveston, TX, January 22-25, 2018. https://ntrs.nasa.gov/search.jsp?R=20170009912 , Jan-2018
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Abstracts for Journals and Proceedings
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Vyas RJ, Murray MC, Predovic M, Lim S, Askin KN, Vizzeri GM, Taibbi G, Mason SS, Zanello SB, Young M, Parsons-Wingerter P. "Analysis by NASA’s VESGEN Software of Retinal Blood Vessels Before and After 70-Day Bed Rest." Human Research Program Investigators’ Workshop (IWS), 'A New Dawn: Enabling Human Space Exploration,' Galveston, TX, January 23-26, 2017. 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. , Jan-2017
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Abstracts for Journals and Proceedings
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Parsons-Wingerter P, Vyas RJ, Murray MC, Predovic M, Lim S, Vizzeri GM, Taibbi G, Mason SS, Zanello SB, Young M. "Mapping by VESGEN of Blood Vessels in the Retina of Astronauts Pre- and Post-Flight to the ISS." Human Research Program Investigators’ Workshop (IWS), 'A New Dawn: Enabling Human Space Exploration,' Galveston Island Convention Center, TX, January 23-26, 2017. 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. https://ntrs.nasa.gov/search.jsp?R=20170009806&hterms=parsons-wingerter&qs=N%3D0%26Ntk%3DAll%26Ntt%3Dparsons-wingerter%26Ntx%3Dmode%2520matchallpartial , Jan-2017
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Abstracts for Journals and Proceedings
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Parsons-Wingerter P, Kao D, Valizadegan H, Martin R, Murray M, Ramesh S, Sekaran S. "VESsel GENeration Analysis (VESGEN): Innovative Vascular Mappings for Astronaut Exploration Health Risks and Human Terrestrial Medicine." NASA Ames Research Center Research and Technology Showcase (ARTS), Moffett Field, CA, September 28, 2017. NASA Ames Research Center Research and Technology Showcase (ARTS), Moffett Field, CA, September 28, 2017. https://ntrs.nasa.gov/search.jsp?R=20170012213&hterms=parsons-wingerter&qs=N%3D0%26Ntk%3DAll%26Ntt%3Dparsons-wingerter%26Ntx%3Dmode%2520matchallpartial , Sep-2017
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Abstracts for Journals and Proceedings
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Parsons-Wingerter P, Vyas RJ, Raghunandan S, Vu AC, Zanello S, Ploutz-Snyder R, Taibbi G, Vizzeri GM. "Analysis by NASA’s VESGEN Software of Vascular Branching in the Human Retina with a Ground-Based Microgravity Analog." Session 223: Imaging Posterior Segment, Presentation 1671-CO127, Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting 'A Vision of Hope,' Seattle WA, May 1-5, 2016. Invest Ophthalmol Vis Sci (ARVO Meeting Abstracts). 2016 Sep;57(12):1671. (Association for Research in Vision and Ophthalmology (ARVO) Annual Meeting 'A Vision of Hope,' Seattle WA, May 1-5, 2016.) https://iovs.arvojournals.org/article.aspx?articleid=2560278&resultClick=1 ; https://ntrs.nasa.gov/search.jsp?R=20160006884 , Sep-2016
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Abstracts for Journals and Proceedings
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Raghunandan S, Vyas RJ, Vu AC, Vizzeri G, Taibbi G, Zanello SB, Ploutz-Snyder R, Zanello SB, Parsons-Wingerter P. "Analysis by NASA’s VESGEN Software of Retinal Blood Vessels Before and After 70-Day Bed Rest: A Retrospective Study." Poster Session A: Visual Impairment and Intracranial Pressure #0645-256, Human Research Program Investigators’ Workshop (IWS), 'Frontiers in Human Space Exploration Research,' Galveston Island Convention Center, TX, February 8-11, 2016. 2016 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 8-11, 2016. https://ntrs.nasa.gov/search.jsp?R=20160002099 , Feb-2016
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Abstracts for Journals and Proceedings
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Vu AC, Raghunandan S, Vyas RJ, Radhakrishnan K, Taibbi G, Vizzeri GM, Grant MB, Chalam KV, Parsons-Wingerter P. "Retinal Image Quality Assessment for Spaceflight-Induced Vision Impairment Study." Space Biomedical Research Session, 31st Annual Meeting, American Society for Gravitational & Space Research (ASGSR), Alexandria VA, November 11-14, 2015. 31st Annual Meeting of the American Society for Gravitational and Space Research, Alexandria, VA, November 11-14, 2015. https://ntrs.nasa.gov/archive/nasa/casi.ntrs.nasa.gov/20160001752.pdf , Nov-2015
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Articles in Peer-reviewed Journals
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Vyas RJ, Young M, Murray MC, Predovic M, Lim S, Jacobs NM, Mason SS, Zanello SB, Taibbi G, Vizzeri G, Parsons-Wingerter P. "Decreased vascular patterning in the retinas of astronaut crew members as new measure of ocular damage in spaceflight-associated neuro-ocular syndrome." Invest Ophthalmol Vis Sci. 2020 Dec 1;61(14):34. https://doi.org/10.1167/iovs.61.14.34 ; PMID: 33372980; PMCID: PMC7774106
, Dec-2020
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Articles in Peer-reviewed Journals
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Lagatuz M, Vyas RJ, Predovic M, Lim S, Jacobs N, Martinho M, Valizadegan H, Kao D, Oza N, Theriot CA, Zanello SB, Taibbi G, Vizzeri G, Dupont M, Grant MB, Lindner DJ, Reinecker HC, Pinhas A, Chui TY, Rosen RB, Moldovan N, Vickerman MB, Radhakrishnan K, Parsons-Wingerter P. "Vascular patterning as integrative readout of complex molecular and physiological signaling by VESsel GENeration Analysis." J Vasc Res. 2021 Jul;58(4):207-30. https://doi.org/10.1159/000514211 ; PMID: 33839725
, Jul-2021
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Articles in Peer-reviewed Journals
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Taibbi G, Young M, Vyas RJ, Murray MC, Lim S, Predovic M, Jacobs NM, Askin KN, Mason SS, Zanello SB, Vizzeri G, Theriot CA, Parsons-Wingerter P. "Opposite response of blood vessels in the retina to 6° head-down tilt and long-duration microgravity." npj Microgravity. 2021 Oct 14;7(1):38. https://doi.org/10.1038/s41526-021-00165-5 ; PMID: 34650071; PMCID: PMC8516890
, Oct-2021
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