Task Progress:
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AIM 1: Develop, refine, and test in space analogs improved ultrasound imaging protocols to enhance kidney stone detection. Our experimental results in ex vivo human kidney stones showed that increasing the acoustic energy delivered to the stone and reducing the ultrasound frequency enhances the color Doppler ultrasound twinkling artifact. On a flexible ultrasound system, twinkling can be further enhanced by increasing the number of cycles and amplitude of the Doppler pulse. Low frequency ultrasound has been shown to enhance twinkling in stones, yet there remains some stones that do not twinkle. We found that internal micro-cracks within the stone contribute to twinkling, in addition to the surface crevice bubbles, but thus far no structural patterns have been identified that might lead to the prediction of stone type with ultrasound.
AIM 2: Determine the effect of breathing gas composition on twinkling in swine. A total of 13 swine have been exposed to elevated levels of carbon dioxide (CO2) in air at 0.8%, 0.54%, and 0.27% (approximately 6, 4, and 2 mm Hg, respectively). The 9 swine initially breathing 100% oxygen (O2) show a statistically significant reduction in twinkling when the swine were exposed to air with elevated CO2 with the degree of decrease in twinkling proportional to the concentration of CO2 (i.e., higher CO2 levels causes more reduction in twinkling: 0.8% > 0.54% > 0.27%). A second set of 4 swine were oscillated between normal air (0.04% CO2) and elevated CO2 at 0.54%. These swine showed a significant decrease in twinkling over the course of the study with only minor increases in twinkling when the exposure was shifted from the elevated CO2 to normal air. The slight increases in twinkling from exposure to normal air were not sufficient to recover from the loss in twinkling from exposure to the elevated CO2. Results from measuring the concentrations of gases in the blood and urine suggest both O2 and CO2 contribute to twinkling as fluctuations from oscillating the respiratory gas and overall trends are observed for both O2 and CO2. The results in swine are supported by the human hyperbaric study where a statistically significant increase in twinkling was observed when subjects breathe 100% O2 at 1.6 ATA (the decompression stop) compared to initial twinkling levels on ambient air.
AIM 3: Determine the role of bacteria in twinkling. Preliminary studies where twinkling was evaluated in fresh kidney stones before sections of the stone and surrounding solution were analyzed for bacteria suggest that bacteria may play a role in twinkling. In the lab, growing two different types of bacteria on sterilized human kidney stones did not induce or increase twinkling. Further testing showed bacteria grown on agar plates did not twinkle. It remains possible that bacteria present in the stone formation process contribute to twinkling, but bacteria, alone or in a biofilm, do not show twinkling with the current ultrasound equipment.
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Bibliography Type:
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Description: (Last Updated: 09/07/2020)
Show Cumulative Bibliography Listing
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Abstracts for Journals and Proceedings
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Bailey M, Dunmire B, Simon J, Maxwell A, Harper J, Sorensen M, Wessells H, Coburn M, Crum L, Khokhlova V, Lingeman J. "Prevention of renal stone complications in space exploration." 2016 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 8-11, 2016. 2016 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 8-11, 2016. , Feb-2016
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Abstracts for Journals and Proceedings
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Bruce M, Cunitz B, Dunmire B, Simon J, Sapozhnikov O, Bailey MR, Thiel J, May PC, Sorensen MD, Harper JD. "Similarities of harmonic Doppler signals from kidney stones and ultrasound contrast agents." Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Abstract Book, p. 70. http://engineering-urology.org/am/31EUS_2016.pdf ; accessed 3/24/17. , May-2016
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Abstracts for Journals and Proceedings
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Cunitz BW, Dunmire B, Haider Y, Simon JC, Sapozhnikov OA, Bailey MR, Thiel J, Maxwell AD, May PC, Sorensen MD, Harper JD. "Increased contrast of stone specific ultrasound imaging in human subjects." Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Abstract Book, p. 35. http://engineering-urology.org/am/31EUS_2016.pdf ; accessed 3/24/17. , May-2016
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Abstracts for Journals and Proceedings
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Dunmire BL, Cunitz BW, Wang Y-N, Simon JC, Maxwell AD, Kreider W, Harper JD, Sorensen MD, Wessells H, Hall MK, Janssen K, Ahn J, Zwaschka TA, Ghanem M, Bruce M, Sapozhnikov OA, Bailey MR. "A phantom and test bed to enhance kidney stone comminution by therapeutic ultrasound." 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. , Jan-2017
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Abstracts for Journals and Proceedings
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Khokhlova V, Schade G, Khokhlova T, Wang Y-N, Simon J, Starr F, Maxwell A, Bailey M, Kreider W. "Pilot in vivo studies on transcutaneous boiling histotripsy in porcine liver and kidney." 5th Joint Meeting of the Acoustical Society of America and the Acoustical Society of Japan, Honolulu, HI, November 28-December 2, 2016. Journal of the Acoustical Society of America. 2016 Oct;40(4):3030. http://doi.org/10.1121/1.4969401 , Oct-2016
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Abstracts for Journals and Proceedings
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Simon JC, Cunitz BW, Sapozhnikov OA, Wang Y-N, Starr F, Thiel J, Holm JR, Sorensen MD, Bailey MR. "Kidney stone detection in space with the color-Doppler ultrasound twinkling artifact." 87th Aerospace Medical Association Annual Meeting, Atlantic City, NJ, April 24-28, 2016. Aerospace Medicine and Human Performance. 2016 Mar;87(3). , Mar-2016
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Abstracts for Journals and Proceedings
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Simon JC, Cunitz BW, Sapozhnikov OA, Wang, Y-N, Dunmire BL, Thiel J, Starr F, Holm JR, Bailey MR. "The effect of respiratory gas composition and pressure on kidney stone detection with the ultrasound twinkling artifact." 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. 2017 NASA Human Research Program Investigators’ Workshop, Galveston, TX, January 23-26, 2017. , Jan-2017
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Abstracts for Journals and Proceedings
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Simon JC, Cunitz BW, Thiel J, Sapozhnikov OA, Holm JR, Dunmire B, Sorensen MD, Bailey MR. "Evidence for trapped bubbles as the cause for the color Doppler ultrasound twinkling artifact." 45th Annual Ultrasound in Industry Association Symposium, Seattle, WA, April 4-6, 2016. 45th Annual Ultrasound in Industry Association Symposium, Seattle, WA, April 4-6, 2016. , Apr-2016
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Abstracts for Journals and Proceedings
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Simon JC, Dunmire B, Cunitz BW, Sapozhnikov OA, Maxwell A, Kreider W, Thiel J, Wang Y-N, Wessells H, Janssen K, Ahn J, Bailey MR. "Development of ultrasonic technologies for management of kidney stones in spaceflight." 88th Aerospace Medical Association Annual Meeting, Denver, CO, April 30-May 4, 2017. Aerospace Medical Association, Abstract Book, April - May, 2017. In press as of March 2017. , Mar-2017
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Abstracts for Journals and Proceedings
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Simon JC, Dunmire B, Cunitz BW, Sapozhnikov OA, Thiel J, Bailey MR, Holm JR. "Color Doppler imaging of in situ human kidney stones in a hyperbaric chamber." 5th Joint Meeting of the Acoustical Society of America and the Acoustical Society of Japan, Honolulu, HI, November 28-December 2, 2016. Journal of the Acoustical Society of America. 2016 Oct;140(4):2983. http://doi.org/10.1121/1.4969238 , Oct-2016
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Abstracts for Journals and Proceedings
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Simon JC, Wang Y-N, Cunitz BW, Sapozhnikov OA, Thiel J, Starr F, Bailey MR. "The effect of carbon dioxide on kidney stone detection with ultrasound." 2016 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 8-11, 2016. 2016 NASA Human Research Program Investigators’ Workshop, Galveston, TX, February 8-11, 2016. , Feb-2016
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Abstracts for Journals and Proceedings
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Simon JC, Wang Y-N, Cunitz BW, Thiel J, Starr F, Liu Z, Bailey MR, Sorensen MD. "The effect of inhaled carbon dioxide on kidney stone detection with ultrasound." Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016. Abstract Book, p. 33. http://engineering-urology.org/am/31EUS_2016.pdf ; accessed 3/24/17. , May-2016
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Abstracts for Journals and Proceedings
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Simon JC, Wang Y-N, Thiel J, Starr F, Bailey MR. "The effect of respiratory gas composition on kidney stone detection with the color Doppler ultrasound twinkling artifact." 3rd Joint Meeting of the Acoustical Society of America and the European Acoustical Society Meeting, Boston, MA, June 25-29, 2017. Journal of the Acoustical Society of America. 2017 May;141(5)3955. Meeting abstract issue, http://dx.doi.org/10.1121/1.4988989 , May-2017
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Articles in Peer-reviewed Journals
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Simon JC, Dunmire B, Bailey MR, Sorensen MD. "Developing complete ultrasonic management of kidney stones for spaceflight." Journal of Space Safety Engineering. 2016 Sep;3(2):50-7. Review. http://dx.doi.org/10.1016/S2468-8967(16)30018-0 , Sep-2016
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Articles in Peer-reviewed Journals
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Simon JC, Wang YN, Cunitz BW, Thiel J, Starr F, Liu Z, Bailey MR. "Effect of carbon dioxide on the twinkling artifact in ultrasound imaging of kidney stones: A pilot study." Ultrasound Med Biol. 2017 May;43(5):877-83. Epub 2017 Feb 9. http://dx.doi.org/10.1016/j.ultrasmedbio.2016.12.010 ; PubMed PMID: 28190622 , May-2017
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Articles in Peer-reviewed Journals
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Simon JC, Sapozhnikov OA, Kreider W, Breshock M, Williams JC, Bailey MR. "The role of trapped bubbles in kidney stone detection with the color Doppler ultrasound twinkling artifact." Phys Med Biol. 2018 Jan 9;63(2):025011. https://doi.org/10.1088/1361-6560/aa9a2f ; PubMed PMID: 29131810; PubMed Central PMCID: PMC5791757 , Jan-2018
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Articles in Peer-reviewed Journals
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Khokhlova TD, Schade GR, Wang YN, Buravkov SV, Chernikov VP, Simon JC, Starr F, Maxwell AD, Bailey MR, Kreider W, Khokhlova VA.
"Pilot in vivo studies on transcutaneous boiling histotripsy in porcine liver and kidney." Sci Rep. 2019 Dec 27;9(1):20176. https://doi.org/10.1038/s41598-019-56658-7 ; PMID: 31882870; PMCID: PMC6934604
, Dec-2019
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Articles in Peer-reviewed Journals
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Simon JC, Holm JR, Thiel J, Dunmire B, Cunitz BW, Bailey MR. "Evidence of microbubbles on kidney stones in humans." Ultrasound Med Biol. 2020 Jul;46(7):1802-7. https://doi.org/10.1016/j.ultrasmedbio.2020.02.010 ; PMID: 32245546; PMCID: PMC7293935
, Jul-2020
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Awards
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Bruce M, Cunitz B, Tremblay-Darveau C, Simon J, Dunmire B, Bailey M. " 'Observations of the Doppler Twinkling Artifact of Kidney Stones Are Bubble Related.' Best Poster Award at the 21st European Symposium on Ultrasound Contrast Imaging, Rotterdam, The Netherlands, January 21-22, 2016." Jan-2016
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Awards
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Cunitz BW, Dunmire B, Haider Y, Simon JC, Sapozhnikov OA, Bailey MR, Thiel J, Maxwell AD, May PC, Sorensen MD, Harper JD. " 'Increased contrast of stone specific ultrasound imaging in human subjects.' Best Paper Award at the Engineering and Urology Society 31st Annual Meeting, American Urological Association, San Diego, CA, May 7, 2016." May-2016
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Awards
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Simon J. "Young Investigator's Travel Grant sponsored by the Women in Acoustics at the 5th Joint Acoustical Society of America and Acoustical Society of Japan Meeting, Honolulu, HI, November 28-December 2, 2016." Nov-2016
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Significant Media Coverage
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Tomlinson SK. "Graduate Program in Acoustics welcomes Julianna Simon to its faculty. Announcement includes descriptions of Dr. Simon's work as NSBRI postdoc awardee." Penn State Graduate Program in Acoustics Announcement, August 2016., Aug-2016
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