Task Progress:
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In prior project years, P53deltaP mice on an FVB/JAX genetic background were obtained and used to create a breeding colony. However, at around 100 days of age, these mice started developing a range of hematologic and solid tumors, including lung tumors, mammary tumors, and subcutaneous tumors. The unexpectedly high rate of tumor formation in untreated animals make P53deltaP mice on an FVB/JAX genetic background unsuitable for studying long-term carcinogenesis from low doses of ionizing radiation. Therefore, we began crossing P53deltaP mice onto a mixed C57BL/6J and 129S4/SvJae background. We expect that this genetic background will lower the number of spontaneous tumors. We are following a breeding strategy, combined with single nucleotide polymorphism (SNP) based assessment of genetic background, to select the mice with the most desirable background. This reduces the number of generations required to create P53deltaP mice onto the mixed C57BL/6J and 129S4/SvJae background. The crossing onto the C57BL/6J background is almost complete and will be followed by crossing onto the 129S4/SvJae background. Then, mice created from the colony need to be housed for at least 200 days to observe spontaneous tumor formation.
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Abstracts for Journals and Proceedings
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Boerma M, Sridharan V, Landes RD, Weil MM. "Gamma-tocotrienol as a countermeasure against high-energy charged particle-induced carcinogenesis." 2021 NASA Human Research Program Investigators’ Workshop, Virtual, February 1-4, 2021. Abstracts. 2021 NASA Human Research Program Investigators’ Workshop, Virtual, February 1-4, 2021. , Feb-2021
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Articles in Peer-reviewed Journals
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Girgis M, Li Y, Jayatilake M, Gill K, Wang S, Makambi K, Sridharan V, Cheema AK. "Short-term metabolic disruptions in urine of mouse models following exposure to low doses of oxygen ion radiation." J Environ Sci Health C Toxicol Carcinog. In special issue on "Overcoming the challenges of space environment," edited by M. Boerma, I. Koturbashhttps. 2021 Apr 27;39(2):234-49. https://doi.org/10.1080/26896583.2020.1868866 ; PMID: 33902388 , Apr-2021
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Articles in Peer-reviewed Journals
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Dissmore T, DeMarco AG, Jayatilake M, Girgis M, Bansal S, Li Y, Mehta K, Sridharan V, Gill K, Bansal S, Tyburski JB, Cheema AK. "Longitudinal metabolic alterations in plasma of rats exposed to low doses of high linear energy transfer radiation." J Environ Sci Health C Toxicol Carcinog. In special issue on "Overcoming the challenges of space environment," edited by M. Boerma, I. Koturbashhttps. 2021 Apr 27;39(2):219-33. https://doi.org/10.1080/26896583.2020.1865027 ; PubMed PMID: 33902389 , Apr-2021
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Articles in Peer-reviewed Journals
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Nemec-Bakk AS, Sridharan V, Landes RD, Singh P, Cao M, Seawright JW, Liu X, Zheng G, Dominic P, Pathak R, Boerma M. "Mitigation of late cardiovascular effects of oxygen ion radiation by gamma-tocotrienol in a mouse model." Life Sci Space Res (Amst). 2021 Nov;31:43-50. https://doi.org/10.1016/j.lssr.2021.07.006 ; PubMed PMID: 34689949; PubMed PMCID: PMC8548672 , Nov-2021
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